Rule-Based Design of Plant Expression Vectors Using GenoCAD

Correction: Rule-Based Design of Plant Expression Vectors Using GenoCAD

The following information is missing from the Competing interests section: Jean Peccoud holds a financial interest in GenoCAD and serves as the company’s CEO. This affiliation does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials. GenoCAD did not provide any funding for this study and was not involved in the design of the research, the collection of da...

Rule-Based Design of Plant Expression Vectors Using GenoCAD

Plant synthetic biology requires software tools to assist on the design of complex multi-genic expression plasmids. Here a vector design strategy to express genes in plants is formalized and implemented as a grammar in GenoCAD, a Computer-Aided Design software for synthetic biology. It includes a library of plant biological parts organized in structural categories and a set of rules describing ...

Construction of Plant Expression Vectors Harboring WRI1 and LPAAT Genes and Its Transformation in Tobacco Plants

In oilseed crops, a number of genes involved in the production of triacylglycerol have been identified that changes in their expression have increase the seed oil content. WRI1 and LPAAT are key genes in this synthetic pathway that their overexpression can increase the oil content. In this study, the expression vectors carrying WRI1 and LPAAT genes were designed and constructed for genetic tran...

Reducing expression size using rule-based integration

This paper describes continuing progress on the development of a repository of transformation rules relevant to indefinite integration. The methodology, however, is not restricted to integration. Several optimization goals are being pursued, including achieving the best form for the output, reducing the size of the repository while retaining its scope, and minimizing the number of steps require...

Runtime verification of programs using rule-based active system